Projekt 04FMTHH15

Assessment of diabetes-induced changes of bone tissue: Experimental identification of fracture risk factors and treatment options in Diabetes Mellitus Type 1 and 2

Ausgangssituation

The project “Assessment of diabetes-induced changes of bone tissue: Experimental identification of fracture risk factors and treatment options in Diabetes Mellitus Type 1 and 2” was funded by the FMTHH as a cooperation between the Department of Osteology and Biomechanics (UKE) and the Institute for Biomechanics (TUHH).

Diabetes Mellitus is a metabolic disease with increasing prevalence affecting several organs in the body including the bone. Patients with both types of diabetes mellitus suffer from an increased fracture risk, which is hardly detectable with common diagnosis techniques, pointing towards an impaired bone material quality.

Zielsetzung

One possible impairment of the diabetic bone quality might be induced by increased accumulation of advanced glycation end-products (AGE) within the bone matrix. Therefore, the aim of this project was to combine the expertise in computed tomography and fluorescence microscopy from the TUHH with the expertise in Fourier-transform infrared microscopy (FTIR) and histomorphometry at the UKE to establish and apply new analysis methods to determine AGE accumulation within the bone matrix.

Vorgehensweise

Primary, bone treated with ribose was analysed with different techniques (high pressure liquid chromatography, fluorescence microscopy, FTIR, and fluorometric assay) to determine AGEs within artificially aged bone which was published by Schmidt et al. (Bone 2017). Further analysis of bone material quality analysis of human diabetic bone tissue were performed and presented at national and international conferences such as the meeting of the European Calcified Tissue Society and the American Society for Bone and Mineral Research.


Beteiligte

Dr. Graeme Campbell, PhD
Projektbearbeitung
Institut für Biomechanik
TUHH

 

Dr. Elizabeth Zimmermann, PhD

Heisenberg Research Group & Bioengineering and Medical Technology Division
c/o Institut für Osteologie und Biomechanik (IOBM)

UKE

Dr. Björn Busse

Heisenberg Research Group & Bioengineering and Medical Technology Division
c/o Institut für Osteologie und Biomechanik (IOBM)

UKE


Publikationsliste

Campbell GM, Picke A-K, Hofbauer C, Busse B, Hofbauer LC, Morlock MM, Using active shape models to quantify impeded skeletal development in an early-onset rat model of type 2 diabetes mellitus. In: The International Bone Minearal Densitomemtry Workshop. Banz, Germany; 2017

Campbell GM, Schmidt FN, Morlock MM, Busse B, The relationship between advanced glycation endproducts and local bone mechanics using fluorescence microscopy and reference point indentation. In: The International Bone Minearal Densitomemtry Workshop. Banz, Germany; 2017

Campbell GM, Picke A-K, Hofbauer C, Busse B, Hofbauer LC, Morlock MM, The effect of early-onset type 2 diabetes mellitus on the development of bone shape in rats. In: 44th European Calcified Tissue Society Congress. Salzburg, Austria; 2017


Folgeprojekte/ Drittmittel

The profound cooperation between the FMTHH laboratories of Prof. Dr. Busse (UKE), Prof. Dr. Morlock (TUHH), Prof. Dr. Schneider (TUHH), and Prof. Dr. Püschel (UKE) led to further research projects and exchange between young scientists.

Subsequent grants are currently funded by the German Research Society (https://gepris.dfg.de/gepris/projekt/394482524), and an Innovative Training Network by the European Union’s Horizon 2020 Marie Skłodowska-Curie (Fidelio – https://www.fidelio-project.eu/).

These projects are focussing on the increased fracture risk in the diabetic bone disease and aim to elucidate the underlying mechanism to allow for improved diagnosis techniques and better personalized treatment options for patients with diabetes mellitus.